The catalytic-dead Pcif1 regulates gene expression and fertility in Drosophila.

ABSTRACT: Eukaryotic mRNAs are modified at the 5' end with a methylated guanosine (m⁷G) that is attached to the transcription start site (TSS) nucleotide. The TSS nucleotide is 2'-O-methylated (Nm) by CMTR1 in organisms ranging from insects to human. In mammals, the TSS adenosine can be further N ⁶ -methylated by RNA polymerase II phosphorylated CTD-interacting factor 1 (PCIF1) to create m⁶Am. Curiously, the fly ortholog of mammalian PCIF1 is demonstrated to be catalytic-dead, and its functions are not known. Here, we show that Pcif1 mutant flies display a reduced fertility which is particularly marked in females. Deep sequencing analysis of Pcif1 mutant ovaries revealed transcriptome changes with a notable increase in expression of

SUBMITTER: Franco G 

PROVIDER: S-EPMC10158991 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature