Intron dynamics reveal principles of gene regulation during the maternal-to-zygotic transition.

ABSTRACT: The maternal-to-zygotic transition (MZT) is a conserved embryonic process in animals where developmental control shifts from the maternal to zygotic genome. A key step in this transition is zygotic transcription, and deciphering the MZT requires classifying newly transcribed genes. However, due to current technological limitations, this starting point remains a challenge for studying many species. Here, we present an alternative approach that characterizes transcriptome changes based solely on RNA-seq data. By combining intron-mapping reads and transcript-level quantification, we characterized transcriptome dynamics during the Drosophila melanogaster MZT. Our approach provides an accessible platform to investigate transcriptome dynamics that can be applied to the MZT in nonmodel org

SUBMITTER: Riemondy K 

PROVIDER: S-EPMC10158999 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature