M6A-ELISA, a simple method for quantifying N6-methyladenosine from mRNA populations.

ABSTRACT: N6-methyladenosine (m6A) is a widely studied and abundant RNA modification. The m6A mark regulates the fate of RNAs in various ways, which in turn drives changes in cell physiology, development, and disease pathology. Over the last decade, numerous methods have been developed to map and quantify m6A sites genome-wide through deep sequencing. Alternatively, m6A levels can be quantified from a population of RNAs using techniques such as liquid chromatography-mass spectrometry or thin layer chromatography. However, many methods for quantifying m6A levels involve extensive protocols and specialized data analysis, and often only a few samples can be handled in a single experiment. Here, we developed a simple method for determining relative m6A levels in mRNA populations from various sour

SUBMITTER: Ensinck I 

PROVIDER: S-EPMC10159001 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature