Serologically D-negative blood donors in Thailand: molecular variants and diagnostic strategy.

ABSTRACT:

Background

Discriminating individuals with "Asian type DEL" from those who are "true D-negative" (D-) among serologically D- donors/patients in Asia would be very valuable, as clinical outcomes are different in these groups. Here we investigated the molecular basis of D-negativity in Thai blood donors, designing a specific strategy for this purpose.

Materials and methods

After routine testing, a total of 1,270 serologically D- blood donors originating from Central, Northeastern and South Thailand underwent analysis of the RHD gene by (i) quantitative multiplex polymerase chain reaction of short fluorescent fragments (QMPSF); (ii) direct sequencing of exon 9 to identify the c.1227G>A variant defining the Asian type DEL allele; and (iii) direct sequencing of the other exons.

SUBMITTER: Nuchnoi P 

PROVIDER: S-EPMC10159805 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature