Antagonism between wild-type and mutant β-catenin controls hepatoblastoma differentiation via fascin-1.

ABSTRACT:

Background & aims

β-catenin is a well-known effector of the Wnt pathway, and a key player in cadherin-mediated cell adhesion. Oncogenic mutations of β-catenin are very frequent in paediatric liver primary tumours. Those mutations are mostly heterozygous, which allows the co-expression of wild-type (WT) and mutated β-catenins in tumour cells. We investigated the interplay between WT and mutated β-catenins in liver tumour cells, and searched for new actors of the β-catenin pathway.

Methods

Using an RNAi strategy in β-catenin-mutated hepatoblastoma (HB) cells, we dissociated the structural and transcriptional activities of β-catenin, which are carried mainly by WT and mutated proteins, respectively. Their impact was characterised using transcriptomic and functional analyses. We

SUBMITTER: Gest C 

PROVIDER: S-EPMC10159820 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature