Reference gene selection for clinical chimeric antigen receptor T-cell product vector copy number assays.

ABSTRACT:

Background aims

Reference genes are an essential part of clinical assays such as droplet digital polymerase chain reaction (ddPCR), which measure the number of copies of vector integrated into genetically engineered cells and the loss of plasmids in reprogrammed cells used in clinical cell therapies. Care should be taken to select reference genes, because it has been discovered that there may be thousands of variations in copy number from genomic segments among different individuals. In addition, within the same person in the context of cancer and other proliferative disorders, substantial parts of the genome also can differ in copy number between cells from diseased and healthy people. The purpose of this study was to identify reference genes that could be used for copy number var

SUBMITTER: Ma J 

PROVIDER: S-EPMC10159958 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature