HyPep: An Open-Source Software for Identification and Discovery of Neuropeptides Using Sequence Homology Search.

ABSTRACT: Neuropeptides are a class of endogenous peptides that have key regulatory roles in biochemical, physiological, and behavioral processes. Mass spectrometry analyses of neuropeptides often rely on protein informatics tools for database searching and peptide identification. As neuropeptide databases are typically experimentally built and comprised of short sequences with high sequence similarity to each other, we developed a novel database searching tool, HyPep, which utilizes sequence homology searching for peptide identification. HyPep aligns de novo sequenced peptides, generated through PEAKS software, with neuropeptide database sequences and identifies neuropeptides based on the alignment score. HyPep performance was optimized using LC-MS/MS measurements of peptide extracts from va

SUBMITTER: Vu NQ 

PROVIDER: S-EPMC10160011 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature