Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule.

ABSTRACT: Of late, numerous microphysiological systems have been employed to model the renal proximal tubule. Yet there is lack of research on refining the functions of the proximal tubule epithelial layer-selective filtration and reabsorption. In this report, pseudo proximal tubule cells extracted from human-induced pluripotent stem cell-derived kidney organoids are combined and cultured with immortalized proximal tubule cells. It is shown that the cocultured tissue is an impervious epithelium that offers improved levels of certain transporters, extracellular matrix proteins collagen and laminin, and superior glucose transport and P-glycoprotein activity. mRNA expression levels higher than those obtained from each cell type were detected, suggesting an anomalous synergistic crosstalk between the tw

SUBMITTER: Banan Sadeghian R 

PROVIDER: S-EPMC10160057 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature