MiR-424(322)-5p targets Ezh1 to inhibit the proliferation and differentiation of myoblasts.

ABSTRACT: The proliferation and differentiation of myoblasts are considered the key biological processes in muscle development and muscle-related diseases, in which the miRNAs involved remain incompletely understood. Previous research reported that miR-424(322)-5p is highly expressed in mouse skeletal muscle. Therefore, C2C12 cells are used as a model to clarify the effect of miR-424(322)-5p on the proliferation and differentiation of myoblasts. The data show that miR-424(322)-5p exhibits a decreasing trend upon myogenic differentiation. Overexpression of miR-424(322)-5p inhibits the proliferation of myoblasts, manifested by downregulation of proliferation marker genes ( CCNB1, CCND2, and CDK4), decreased percentage of EdU ⁺ cells, and reduced cell viability. In contr

SUBMITTER: Yue Y 

PROVIDER: S-EPMC10160230 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature