Diversity and heterogeneity in human breast cancer adipose tissue revealed at single-nucleus resolution.

ABSTRACT:

Introduction

There is increasing awareness of the role of adipose tissue in breast cancer occurrence and development, but no comparison of adipose adjacent to breast cancer tissues and adipose adjacent to normal breast tissues has been reported.

Methods

Single-nucleus RNA sequencing (snRNA-seq) was used to analyze cancer-adjacent and normal adipose tissues from the same breast cancer patient to characterize heterogeneity. SnRNA-seq was performed on 54513 cells from six samples of normal breast adipose tissue (N) distant from the tumor and tumor-adjacent adipose tissue (T) from the three patients (all surgically resected).

Results and discussion

Significant diversity was detected in cell subgroups, differentiation status and, gene expression profiles. Breast cancer ind

SUBMITTER: Tang L 

PROVIDER: S-EPMC10160491 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature