Analysis of sperm separation protocols for isolating cryopreserved human spermatozoa.

ABSTRACT:

Graphical abstract

Abstract

Sperm cryopreservation is a valuable tool for the long-term preservation of male fertility. Thus, determining the optimal technique for isolating spermatozoa post-thaw is vital to ensure recovery of the highest quality spermatozoa with minimal iatrogenic damage. This not only enhances the chances of successful conception but also reduces the risk of genetic damage in the embryo. To address this issue, human semen samples were cryopreserved using a slow freezing protocol and Quinn's Advantage™ Sperm Freeze medium. The samples were subsequently thawed and subjected to three types of sperm isolation procedures: direct swim-up, density gradient centrifugation, and electrophoretic separation using the Felix™ device. Cryopreservation led to the anticip

SUBMITTER: Hungerford AJ 

PROVIDER: S-EPMC10160538 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature