Protocol for indirect and direct co-culture between human cancer cells and endothelial cells.

ABSTRACT: The cross talk between cancer cells and endothelial cells (ECs) within the tumor microenvironment plays a critical role in tumor progression, recurrence, and cancer stemness. Here, we present a protocol containing two in vitro approaches to study such interactions. We first describe an indirect co-culture system to study the regulation of stemness markers in cancer cells by secreted factors from ECs. We then detail a direct co-culture system to study juxtracrine communications between the cell types. For complete details on the use and execution of this protocol, please refer to Sewell-Loftin et al.¹ and Guo et al.².

SUBMITTER: Guo Y 

PROVIDER: S-EPMC10160801 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature