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Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites.

ABSTRACT: Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022).¹.

SUBMITTER: Ibarra-Meneses AV

PROVIDER: S-EPMC10160803 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites.

Ibarra-Meneses Ana Victoria AV Amin Atia A Dong George G Olivier Martin M Langlais David D Fernandez-Prada Christopher C

STAR protocols 20230421 2

Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protoco ...[more]

PMID: 37087735

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