Precise, Orthogonal Remote-Control of Cell-Free Systems Using Photocaged Nucleic Acids.

ABSTRACT: Cell-free expression of a gene to protein has become a vital tool in nanotechnology and synthetic biology. Remote-control of cell-free systems with multiple, orthogonal wavelengths of light would enable precise, noninvasive modulation, opening many new applications in biology and medicine. While there has been success in developing ON switches, the development of OFF switches has been lacking. Here, we have developed orthogonally light-controlled cell-free expression OFF switches by attaching nitrobenzyl and coumarin photocages to antisense oligonucleotides. These light-controlled OFF switches can be made from commercially available oligonucleotides and show a tight control of cell-free expression. Using this technology, we have demonstrated orthogonal degradation of two different mRNAs, d

SUBMITTER: Mazzotti G 

PROVIDER: S-EPMC10161223 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature