Boosting the epoxidation of squalene to produce triterpenoids in Saccharomyces cerevisiae.

ABSTRACT:

Background

Polycyclic triterpenoids (PTs) are common in plants, and have attracted considerable interest due to their remarkable biological activities. Currently, engineering the ergosterol synthesis pathway in Saccharomyces cerevisiae is a safe and cost-competitive way to produce triterpenoids. However, the strict regulation of ERG1 involved in the epoxidation of squalene limits the triterpenoid production.

Results

In this study, we found that the decrease in ERG7 protein level could dramatically boost the epoxidation of squalene by improving the protein stability of ERG1. We next explored the potential factors that affected the degradation process of ERG1 and confirmed that ERG7 was involved in the degradation process of ERG1. Subsequently, expression of four different tri

SUBMITTER: Du MM 

PROVIDER: S-EPMC10161426 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature