Genetic labeling reveals spatial and cellular expression pattern of neuregulin 1 in mouse brain.

ABSTRACT:

Background

Where the gene is expressed determines the function of the gene. Neuregulin 1 (Nrg1) encodes a tropic factor and is genetically linked with several neuropsychiatry diseases such as schizophrenia, bipolar disorder and depression. Nrg1 has broad functions ranging from regulating neurodevelopment to neurotransmission in the nervous system. However, the expression pattern of Nrg1 at the cellular and circuit levels in rodent brain is not full addressed.

Methods

Here we used CRISPR/Cas9 techniques to generate a knockin mouse line (Nrg1^Cre/+) that expresses a P2A-Cre cassette right before the stop codon of Nrg1 gene. Since Cre recombinase and Nrg1 are expressed in the same types of cells in Nrg1^Cre/+ mice, the Nrg1 expression pattern can be revealed

SUBMITTER: Ding CY 

PROVIDER: S-EPMC10161477 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature