Development of a hookworm egg hatching assay to determine the ovicidal effects of anthelminthics.

ABSTRACT:

Background

Few anthelminthics are currently available, manifesting the urgent need for new treatment options. In vitro profiling of current anthelminthics against larval and adult stage helminths displayed varying effects on closely related worm species and between life stages of the same species. Conversely, limited research has been performed on the egg stage of human hookworms, and the effects of investigational compounds on the egg stage are not routinely assessed.

Methods

We profiled the development and hatching of Heligmosomoides polygyrus, Ancylostoma duodenale and Necator americanus eggs isolated from rodent faeces in liquid media with various nutrient levels, osmolar concentrations, and acidities in dependence on incubation temperature and light exposure. Incubation

SUBMITTER: Easland E 

PROVIDER: S-EPMC10161531 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature