A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines.

ABSTRACT:

Background

Multiple myeloma (MM) is the second most common hematologic neoplasm which is characterized by proliferation and infiltration of plasmatic cells in the bone marrow. Currently, MM is considered incurable due to resistance to treatment. The CRISPR/Cas9 system has emerged as a powerful tool for understanding the role of different genetic alterations in the pathogenesis of hematologic malignancies in both cell lines and mouse models. Despite current advances of gene editing tools, the use of CRISPR/Cas9 technology for gene editing of MM have not so far been extended. In this work, we want to repress Rnd3 expression, an atypical Rho GTPase involved in several cellular processes, in MM cell lines using a CRISPR interference strategy.

Results

We have designed different g

SUBMITTER: Escriva-Fernandez J 

PROVIDER: S-EPMC10161638 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature