JINXED: just in time crystallization for easy structure determination of biological macromolecules.

ABSTRACT: Macromolecular crystallography is a well established method in the field of structural biology and has led to the majority of known protein structures to date. After focusing on static structures, the method is now under development towards the investigation of protein dynamics through time-resolved methods. These experiments often require multiple handling steps of the sensitive protein crystals, e.g. for ligand-soaking and cryo-protection. These handling steps can cause significant crystal damage, and hence reduce data quality. Furthermore, in time-resolved experiments based on serial crystallography, which use micrometre-sized crystals for short diffusion times of ligands, certain crystal morphologies with small solvent channels can prevent sufficient ligand diffusion. Described here is

SUBMITTER: Henkel A 

PROVIDER: S-EPMC10161778 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature