Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging.

ABSTRACT: Fluorescence lifetime imaging microscopy (FLIM) and spectral imaging are two broadly applied methods for increasing dimensionality in microscopy. However, their combination is typically inefficient and slow in terms of acquisition and processing. By integrating technological and computational advances, we developed a robust and unbiased spectral FLIM (S-FLIM) system. Our method, Phasor S-FLIM, combines true parallel multichannel digital frequency domain electronics with a multidimensional phasor approach to extract detailed and precise information about the photophysics of fluorescent specimens at optical resolution. To show the flexibility of the Phasor S-FLIM technology and its applications to the biological and biomedical field, we address four common, yet challenging, problems: the bli

SUBMITTER: Scipioni L 

PROVIDER: S-EPMC10161785 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature