Self-Assembled Monolayers As a Tool to Investigate the Effect of Surface Chemistry on Protein Nucleation.

ABSTRACT: Modified surfaces like siliconized glass are commonly used to support protein crystallization and facilitate obtaining crystals. Over the years, various surfaces have been proposed to decrease the energetic penalty required for consistent protein clustering, but scarce attention has been paid to the underlying mechanisms of interactions. Here, we propose self-assembled monolayers that are surfaces exposing fine-tuned moieties with a very regular topography and subnanometer roughness, as a tool to unveil the interaction between proteins and functionalized surfaces. We studied the crystallization of three model proteins having progressively narrower metastable zones, i.e., lysozyme, catalase, and proteinase K, on monolayers exposing thiol, methacrylate, and glycidyloxy groups. Thanks to comp

SUBMITTER: Artusio F 

PROVIDER: S-EPMC10162442 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature