DNA ultra-sensitive quantification, a technology for studying HIV unintegrated linear DNA.

ABSTRACT: Unintegrated HIV DNA represents between 20% and 35% of the total viral DNA in infected patients. Only the linear forms (unintegrated linear DNAs [ULDs]) can be substrates for integration and for the completion of a full viral cycle. In quiescent cells, these ULDs may be responsible for pre-integrative latency. However, their detection remains difficult due to the lack of specificity and sensitivity of existing techniques. We developed an ultra-sensitive, specific, and high-throughput technology for ULD quantification called DUSQ (DNA ultra-sensitive quantification) combining linker-mediated PCR and next-generation sequencing (NGS) using molecular barcodes. Studying cells with different activity levels, we determined that the ULD half-life goes up to 11 days in resting CD4⁺ T cel

SUBMITTER: Roux HM 

PROVIDER: S-EPMC10162948 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature