An inducible model for genetic manipulation and fate-tracing of PDGFRβ-expressing fibrogenic cells in the liver.

ABSTRACT: Myofibroblasts are the source of extracellular matrix protein during liver fibrogenesis. Fibroblasts, hepatic stellate cells (HSCs) and vascular smooth muscle cells are mesenchymal subpopulations in the liver that are characterized by the expression of PDGFRβ and contribute to the pool of these myofibroblasts. Conditional knockout models are important to better understand the function of specific liver cell populations including mesenchymal cells. While there is a limited number of constitutive mouse models for liver mesenchymal cell specific transgene expression, there is no established model for inducible gene targeting in HSCs or PDGFRβ-expressing mesenchymal cell populations in the liver. To address this, we investigated whether the tamoxifen inducible PDGFRβ-P2A-CreER^T2 mou

SUBMITTER: Hamberger F 

PROVIDER: S-EPMC10162963 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature