TCR sequencing and cloning methods for repertoire analysis and isolation of tumor-reactive TCRs.

ABSTRACT: T cell receptor (TCR) technologies, including repertoire analyses and T cell engineering, are increasingly important in the clinical management of cellular immunity in cancer, transplantation, and other immune diseases. However, sensitive and reliable methods for repertoire analyses and TCR cloning are still lacking. Here, we report on SEQTR, a high-throughput approach to analyze human and mouse repertoires that is more sensitive, reproducible, and accurate as compared with commonly used assays, and thus more reliably captures the complexity of blood and tumor TCR repertoires. We also present a TCR cloning strategy to specifically amplify TCRs from T cell populations. Positioned downstream of single-cell or bulk TCR sequencing, it allows time- and cost-effective discovery, cloning, screeni

SUBMITTER: Genolet R 

PROVIDER: S-EPMC10163020 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature