Multilineage contribution of CD34⁺ cells in cardiac remodeling after ischemia/reperfusion injury.

ABSTRACT: The ambiguous results of multiple CD34⁺ cell-based therapeutic trials for patients with heart disease have halted the large-scale application of stem/progenitor cell treatment. This study aimed to delineate the biological functions of heterogenous CD34⁺ cell populations and investigate the net effect of CD34⁺ cell intervention on cardiac remodeling. We confirmed, by combining single-cell RNA sequencing on human and mouse ischemic hearts and an inducible Cd34 lineage-tracing mouse model, that Cd34⁺ cells mainly contributed to the commitment of mesenchymal cells, endothelial cells (ECs), and monocytes/macrophages during heart remodeling with distinct pathological functions. The Cd34⁺-lineage-activated mesenchymal cells were responsible f

SUBMITTER: Xie J 

PROVIDER: S-EPMC10163140 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature