Voltage sensor current, SR Ca²⁺ release, and Ca²⁺ channel current during trains of action potential-like depolarizations of skeletal muscle fibers.

ABSTRACT: In skeletal muscle, Ca_V 1.1 serves as the voltage sensor for both excitation-contraction coupling (ECC) and L-type Ca²⁺ channel activation. We have recently adapted the technique of action potential (AP) voltage clamp (APVC) to monitor the current generated by the movement of intramembrane voltage sensors (I_Q ) during single imposed transverse tubular AP-like depolarization waveforms (I_QAP ). We now extend this procedure to monitoring I_QAP , and Ca²⁺ currents during trains of tubular AP-like waveforms in adult murine skeletal muscle fibers, and compare them with the trajectories of APs and AP-induced Ca²⁺ release measured in other fibers using field stimulation and optical probes. The AP waveform remains relativel

SUBMITTER: Bibollet H 

PROVIDER: S-EPMC10163276 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature