Dataset Information

Tissue-like cultured fish fillets through a synthetic food pipeline.

ABSTRACT: Tissue-like cultured meats of some livestock have successfully been established by different approaches. However, the production of a structure similar to fish fillets is still challenging. Here, we develop tissue-like cultured fish fillets by assembly of large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibition of Tgf-β and Notch signals significantly promoted myogenic differentiation of piscine satellite cells (PSCs). The mixture of fish gelatin and sodium alginate combined with a p53 inhibitor and a Yap activator supported PSC viability and proliferation. Based on the texture of fish muscle tissue, a 3D scaffold was constructed by gelatin-based gel mixed with PSCs. After proliferation and differentiation, the muscle scaffold was filled with cultured piscine adipocytes. Finally, tissue-like fish fillets with 20 × 12 × 4 mm were formed, consisting of 5.67 × 10⁷ muscles and 4.02 × 10⁷ adipocytes. The biomanufacture of tissue-like cultured fish fillet here could be a promising technology to customize meat production with high fidelity.

SUBMITTER: Xu E

PROVIDER: S-EPMC10164169 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Tissue-like cultured fish fillets through a synthetic food pipeline.

Xu Enbo E Niu Ruihao R Lao Jihui J Zhang Shengliang S Li Jie J Zhu Yiyuan Y Shi Huimin H Zhu Qingqing Q Chen Yijian Y Jiang Yuyan Y Wang Wenjun W Yin Jun J Chen Qihe Q Huang Xiao X Chen Jun J Liu Donghong D

NPJ science of food 20230506 1

Tissue-like cultured meats of some livestock have successfully been established by different approaches. However, the production of a structure similar to fish fillets is still challenging. Here, we develop tissue-like cultured fish fillets by assembly of large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibition of Tgf-β and Notch signals significantly promoted myogenic differentiation of piscine satellite cells (PSCs). The mixture of fish gelatin and sodium alginate comb ...[more]

PMID: 37149658

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Dataset Information

Tissue-like cultured fish fillets through a synthetic food pipeline.

Publications

Tissue-like cultured fish fillets through a synthetic food pipeline.

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