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Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei.

ABSTRACT: Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization.

SUBMITTER: Zhao J

PROVIDER: S-EPMC10164179 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei.

Zhao Jie J Zhang Meng M Hui Wenyan W Zhang Yue Y Wang Jing J Wang Shaojing S Kwok Lai-Yu LY Kong Jian J Zhang Heping H Zhang Wenyi W

Nature communications 20230506 1

Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, sho ...[more]

PMID: 37149616

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