Tuning the surface charge of phospholipid bilayers inhibits insulin fibrilization.

ABSTRACT: The interactions between proteins and materials, in particular lipid bilayers, have been studied extensively for their relevance in diseases and for the formulation of protein-based therapeutics and vaccines. However, the precise rules by which material properties induce favorable or unfavorable structural states in biomolecules are incompletely understood, and as a result, the rational design of materials remains challenging. Here, we investigated the influence of lipid bilayers (in the form of small unilamellar vesicles) on the formation of insulin amyloid fibrils using a fibril-specific assay (thioflavin T), polyacrylamide gel electrophoresis, and circular dichroism spectroscopy. Lipid bilayers composed of equal mixtures of cationic and anionic lipids effectively inhibited fibril format

SUBMITTER: Reichelderfer VT 

PROVIDER: S-EPMC10164472 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature