Protodioscin protects porcine oocytes against H2O2-induced oxidative stress during in vitro maturation.

ABSTRACT:

Objective

The present study investigated whether protodioscin (PD), a steroidal saponin mainly found in rhizome of Dioscorea species, alleviates oxidative stress-induced damage of porcine oocytes during in vitro maturation.

Methods

Oocytes were treated with different concentrations of PD (0, 1, 10, 100, and 200 μM) in the presence of 200 μM H2O2 during in vitro maturation. Following maturation, spindle morphology and mitogen-activated protein kinase activity was assessed along with reactive oxygen species level, GSH activity, and mRNA expression of endogenous antioxidant genes at the MII stage. On the day 7 after parthenogenetic activation, blastocyst formation rate was calculated and the quality of embryo and mRNA expression of development-related genes was evaluated.

Re

SUBMITTER: Kim SH 

PROVIDER: S-EPMC10164477 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature