Novel polymorphisms of dopa decarboxylase gene and their association with lamb quality traits in Indonesian sheep.

ABSTRACT:

Objective

This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep.

Methods

The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse

SUBMITTER: Harahap RS 

PROVIDER: S-EPMC10164533 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature