Cryo-EM reconstruction of the human 40S ribosomal subunit at 2.15 A resolution.

ABSTRACT: The chemical modification of ribosomal RNA and proteins is critical for ribosome assembly, for protein synthesis and may drive ribosome specialisation in development and disease. However, the inability to accurately visualise these modifications has limited mechanistic understanding of the role of these modifications in ribosome function. Here we report the 2.15 Å resolution cryo-EM reconstruction of the human 40S ribosomal subunit. We directly visualise post-transcriptional modifications within the 18S rRNA and four post-translational modifications of ribosomal proteins. Additionally, we interpret the solvation shells in the core regions of the 40S ribosomal subunit and reveal how potassium and magnesium ions establish both universally conserved and eukaryote-specific coordination to prom

SUBMITTER: Pellegrino S 

PROVIDER: S-EPMC10164566 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature