METTL3 regulates alternative splicing of cell cycle-related genes via crosstalk between mRNA m⁶A modifications and splicing factors.

ABSTRACT: N⁶-methyladenosine (m⁶A) modification in RNA affects various aspects of RNA metabolism and regulates gene expression. This modification is modulated by many regulatory proteins, such as m⁶A methyltransferases (writers), m⁶A demethylases (erasers), and m⁶A-binding proteins (readers). Previous studies have suggested that alterations in m⁶A regulatory proteins induce genome-wide alternative splicing in many cancer cells. However, the functional effects and molecular mechanisms of m⁶A-mediated alternative splicing have not been fully elucidated. To understand the consequences of this modification on RNA splicing in cancer cells, we performed RNA sequencing and analyzed alternative splicing patterns in METTL3-knockdown

SUBMITTER: Kim Y 

PROVIDER: S-EPMC10164804 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature