High sensitivity and low-cost flavin luciferase (FLUX^Vc)-based reporter gene for mammalian cell expression.

ABSTRACT: Luciferase-based gene reporters generating bioluminescence signals are important tools for biomedical research. Amongst the luciferases, flavin-dependent enzymes use the most economical chemicals. However, their applications in mammalian cells are limited due to their low signals compared to other systems. Here, we constructed Flavin Luciferase from Vibrio campbellii (Vc) for Mammalian Cell Expression (FLUX^Vc) by engineering luciferase from V. campbellii (the most thermostable bacterial luciferase reported to date) and optimizing its expression and reporter assays in mammalian cells which can improve the bioluminescence light output by >400-fold as compared to the nonengineered version. We found that the FLUX^Vc reporter gene can be overexpressed in various cell lines

SUBMITTER: Phonbuppha J 

PROVIDER: S-EPMC10164909 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature