Serial intravital 2-photon microscopy and analysis of the kidney using upright microscopes.

ABSTRACT: Serial intravital 2-photon microscopy of the kidney and other abdominal organs is a powerful technique to assess tissue function and structure simultaneously and over time. Thus, serial intravital microscopy can capture dynamic tissue changes during health and disease and holds great potential to characterize (patho-) physiological processes with subcellular resolution. However, successful image acquisition and analysis require significant expertise and impose multiple potential challenges. Abdominal organs are rhythmically displaced by breathing movements which hamper high-resolution imaging. Traditionally, kidney intravital imaging is performed on inverted microscopes where breathing movements are partly compensated by the weight of the animal pressing down. Here, we present a custom and

SUBMITTER: Sardella D 

PROVIDER: S-EPMC10164931 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature