Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution.

ABSTRACT: Background: The toxic gas carbon monoxide (CO) is abundantly present in synthesis gas (syngas) and certain industrial waste gases that can serve as feedstocks for the biological production of industrially significant chemicals and fuels. For efficient bacterial growth to occur, and to increase productivity and titres, a high resistance to the gas is required. The aerobic bacterium Cupriavidus necator H16 can grow on CO₂ + H₂, although it cannot utilise CO as a source of carbon and energy. This study aimed to increase its CO resistance through adaptive laboratory evolution. Results: To increase the tolerance of C. necator to CO, the organism was continually subcultured in the presence of CO both heterotrophically and autotrophically. Ten ind

SUBMITTER: Wickham-Smith C 

PROVIDER: S-EPMC10164946 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature