Regulation of Syk activity by antiviral adaptor MAVS in FcεRI signaling pathway.

ABSTRACT:

Background

Mast cells are the major effector cell type for IgE-mediated allergic reactions. Recent studies revealed a role for mast cells in orchestrating the host response to viral infections.

Objective

We studied the relationship between FcεRI (high-affinity IgE receptor) and RIG-I-like receptor (RLR)-mediated antiviral signaling pathways.

Methods

Mast cells (BMMCs) were cultured from bone marrow cells from mice deficient in MAVS or other RLR signaling molecules. MAVS expression was restored by retroviral transduction of MAVS-deficient BMMCs. These cells were stimulated with IgE and antigen and their activation (degranulation and cytokine production/secretion) was quantified. FcεRI-mediated signaling events such as protein phosphorylation and Ca²⁺ flux we

SUBMITTER: Kawakami Y 

PROVIDER: S-EPMC10165108 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature