Terminal fucosylation of haptoglobin in cancer-derived exosomes during cholangiocarcinoma progression.

ABSTRACT:

Background

Cholangiocarcinoma (CCA) is a silent tumor with a high mortality rate due to the difficulty of early diagnosis and prediction of recurrence even after timely surgery. Serologic cancer biomarkers have been used in clinical practice, but their low specificity and sensitivity have been problematic. In this study, we aimed to identify CCA-specific glycan epitopes that can be used for diagnosis and to elucidate the mechanisms by which glycosylation is altered with tumor progression.

Methods

The serum of patients with various cancers was fractioned into membrane-bound and soluble components using serial ultracentrifugation. Lectin blotting was conducted to evaluate glycosylation. Proteins having altered glycosylation were identified using proteomic analysis and further

SUBMITTER: Choi H 

PROVIDER: S-EPMC10165115 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature