The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity, and interaction with 14-3-3 proteins.

ABSTRACT: Potassium is an essential intracellular ion, and a sufficient intracellular concentration of it is crucial for many processes; therefore it is fundamental for cells to precisely regulate K⁺ uptake and efflux through the plasma membrane. The uniporter Trk1 is a key player in K⁺ acquisition in yeasts. The TRK1 gene is expressed at a low and stable level; thus the activity of the transporter needs to be regulated at a posttranslational level. S. cerevisiae Trk1 changes its activity and affinity for potassium ion quickly and according to both internal and external concentrations of K⁺, as well as the membrane potential. The molecular basis of these changes has not been elucidated, though phosphorylation is thought to play an important role. In thi

SUBMITTER: Masaryk J 

PROVIDER: S-EPMC10165143 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature