Combinatorial drug screening on 3D Ewing sarcoma spheroids using droplet-based microfluidics.

ABSTRACT: Culturing and screening cells in microfluidics, particularly in three-dimensional formats, has the potential to impact diverse areas from fundamental biology to cancer precision medicine. Here, we use a platform based on anchored droplets for drug screening. The response of spheroids of Ewing sarcoma (EwS) A673 cells to simultaneous or sequential combinations of etoposide and cisplatin was evaluated. This was done by culturing spheroids of EwS cells inside 500 nL droplets then merging them with secondary droplets containing fluorescent-barcoded drugs at different concentrations. Differences in EwS spheroid growth and viability were measured by microscopy. After drug exposure such measurements enabled estimation of their IC50 values, which were in agreement with values obtained in standard

SUBMITTER: Fevre R 

PROVIDER: S-EPMC10165258 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature