Oxidation Driven Reversal of PIP₂-dependent Gating in GIRK2 Channels.

ABSTRACT: Physiological activity of G protein gated inward rectifier K⁺ (GIRK, Kir3) channel, dynamically regulated by three key ligands, phosphoinositol-4,5-bisphosphate (PIP₂), Gβγ, and Na⁺, underlies cellular electrical response to multiple hormones and neurotransmitters in myocytes and neurons. In a reducing environment, matching that inside cells, purified GIRK2 (Kir3.2) channels demonstrate low basal activity, and expected sensitivity to the above ligands. However, under oxidizing conditions, anomalous behavior emerges, including rapid loss of PIP₂ and Na⁺-dependent activation and a high basal activity in the absence of any agonists, that is now paradoxically inhibited by PIP₂. Mutagenesis identifies two cysteine residues (C65 a

SUBMITTER: Lee SJ 

PROVIDER: S-EPMC10165546 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature