TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation.

ABSTRACT:

Background

Hepatic ischemia/reperfusion (I/R) injury is one of the major pathological processes associated with various liver surgeries. However, there is still a lack of strategies to protect against hepatic I/R injury because of the unknown underlying mechanism. The present study aimed to identify a potential strategy and provide a fundamental experimental basis for treating hepatic I/R injury.

Method

A classic 70% ischemia/reperfusion injury was established. Immunoprecipitation was used to identify direct interactions between proteins. The expression of proteins from different subcellular localizations was detected by Western blotting. Cell translocation was directly observed by immunofluorescence. HE, TUNEL and ELISA were performed for function tests.

Result

We re

SUBMITTER: Yang H 

PROVIDER: S-EPMC10165779 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature