Trace amine-associated receptor 1 regulation of Kv1.4 channels in trigeminal ganglion neurons contributes to nociceptive behaviors.

ABSTRACT:

Background

Trace amines, such as tyramine, are endogenous amino acid metabolites that have been hypothesized to promote headache. However, the underlying cellular and molecular mechanisms remain unknown.

Methods

Using patch-clamp recording, immunostaining, molecular biological approaches and behaviour tests, we elucidated a critically functional role of tyramine in regulating membrane excitability and pain sensitivity by manipulating Kv1.4 channels in trigeminal ganglion (TG) neurons.

Results

Application of tyramine to TG neurons decreased the A-type K⁺ current (I_A) in a manner dependent on trace amine-associated receptor 1 (TAAR1). Either siRNA knockdown of Gαo or chemical inhibition of βγ subunit (G_βγ) signaling abrogated the respons

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10165857 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature