Amyposomes, a nanotechnological chaperone with anti-amyloidogenic activity.

ABSTRACT:

Aim

The effect of liposomes bi-functionalized with phosphatidic acid and with a synthetic peptide derived from human apolipoprotein E has been evaluated on the aggregation features of different amyloidogenic proteins: human Amyloid β1-40 (Aβ_1-40), transthyretin (TTR) variant S52P, human β2microglobulin (β2m) variants ΔN6 and D76N, Serum Amyloid A (SAA).

Methods

The formation of fibrillar aggregates of the proteins was investigated by ThioflavinT fluorescence assay and validated by Atomic Force Microscopy.

Results

The results show that liposomes are preventing the transition of non-aggregated forms to the fibrillar state, with stronger effects on Aβ_1-40, β2m ΔN6 and SAA. Liposomes also induce disaggregation of the amyloid aggregates of all the pro

SUBMITTER: Re F 

PROVIDER: S-EPMC10165933 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature