Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in Scleroderma Patients.

ABSTRACT:

Objective

Patients with diffuse cutaneous systemic sclerosis (dcSSc) display a complex clinical phenotype. Transcriptional profiling of whole blood or tissue from patients are affected by changes in cellular composition that drive gene expression and an inability to detect minority cell populations. We undertook this study to focus on the 2 main subtypes of circulating monocytes, classical monocytes (CMs) and nonclassical monocytes (NCMs) as a biomarker of SSc disease severity.

Methods

SSc patients were recruited from the Prospective Registry for Early Systemic Sclerosis. Clinical data were collected, as well as peripheral blood for isolation of CMs and NCMs. Age-, sex-, and race-matched healthy volunteers were recruited as controls. Bulk macrophages were isolated from the s

SUBMITTER: Makinde HM 

PROVIDER: S-EPMC10165944 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature