High-throughput profiling of histone post-translational modifications and chromatin modifying proteins by reverse phase protein array.

ABSTRACT: Epigenetic variation plays a significant role in normal development and human diseases including cancer, in part through post-translational modifications (PTMs) of histones. Identification and profiling of changes in histone PTMs, and in proteins regulating PTMs, are crucial to understanding diseases, and for discovery of epigenetic therapeutic agents. In this study, we have adapted and validated an antibody-based reverse phase protein array (RPPA) platform for profiling 20 histone PTMs and expression of 40 proteins that modify histones and other epigenomic regulators. The specificity of the RPPA assay for histone PTMs was validated with synthetic peptides corresponding to histone PTMs and by detection of histone PTM changes in response to inhibitors of histone modifier proteins in cell cu

SUBMITTER: Wang X 

PROVIDER: S-EPMC10165948 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature