Discovery of the Xenon-Protein Interactome Using Large-Scale Measurements of Protein Folding and Stability.

ABSTRACT: The intermolecular interactions of noble gases in biological systems are associated with numerous biochemical responses, including apoptosis, inflammation, anesthesia, analgesia, and neuroprotection. The molecular modes of action underlying these responses are largely unknown. This is in large part due to the limited experimental techniques to study protein-gas interactions. The few techniques that are amenable to such studies are relatively low-throughput and require large amounts of purified proteins. Thus, they do not enable the large-scale analyses that are useful for protein target discovery. Here, we report the application of stability of proteins from rates of oxidation (SPROX) and limited proteolysis (LiP) methodologies to detect protein-xenon interactions on the proteomic scale us

SUBMITTER: Wiebelhaus N 

PROVIDER: S-EPMC10166008 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature