Using DNA metabarcoding to assess insect diversity in citrus orchards.

ABSTRACT:

Background

DNA metabarcoding is rapidly emerging as a cost-effective approach for large-scale biodiversity assessment and pest monitoring. The current study employed metabarcoding to assess insect diversity in citrus orchards in Ganzhou City, Jiangxi, China in both 2018 and 2019. Insects were sampled using Malaise traps deployed in three citrus orchards producing a total of 43 pooled monthly samples.

Methods

The Malaise trap samples were sequenced following DNA metabarcoding workflow. Generated sequences were curated and analyzed using two cloud databases and analytical platforms, the barcode of life data system (BOLD) and multiplex barcode research and visualization environment (mBRAVE).

Results

These platforms assigned the sequences to 2,141 barcode index numbers (B

SUBMITTER: Liu C 

PROVIDER: S-EPMC10166080 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature