Modeling human pregastrulation development by 3D culture of blastoids generated from primed-to-naive transitioning intermediates.

ABSTRACT: Human pluripotent stem cells provide an inexhaustible model to study human embryogenesis in vitro. Recent studies have provided diverse models to generate human blastoids by self-organization of different pluripotent stem cells or somatic reprogramming intermediates. However, whether blastoids can be generated from other cell types or whether they can recapitulate postimplantation development in vitro is unknown. Here, we develop a strategy to generate human blastoids from heterogeneous intermediates with epiblast, trophectoderm, and primitive endoderm signatures of the primed-to-naïve conversion process, which resemble natural blastocysts in morphological architecture, composition of cell lineages, transcriptome, and lineage differentiation potential. In addition, these blastoids reflect

SUBMITTER: Tu Z 

PROVIDER: S-EPMC10166166 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature