Accurate prostate cancer detection based on enrichment and characterization of prostate cancer specific circulating tumor cells.

ABSTRACT:

Background

The low specificity of serum PSA resulting in the inability to effectively differentiate prostate cancer from benign prostate conditions is a persistent clinical challenge. The low sensitivity of serum PSA results in false negatives and can miss high-grade prostate cancers. We describe a non-invasive test for detection of prostate cancer based on functional enrichment of prostate adenocarcinoma associated circulating tumor cells (PrAD-CTCs) from blood samples followed by their identification by immunostaining for pan-cytokeratins (PanCK), prostate specific membrane antigen (PSMA), alpha methyl-acyl coenzyme-A racemase (AMACR), epithelial cell adhesion molecule (EpCAM), and common leucocyte antigen (CD45).

Methods

Analytical validation studies were performed to est

SUBMITTER: Limaye S 

PROVIDER: S-EPMC10166919 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature